El microbioma intestinal puede afectar varios aspectos tanto del comportamiento como de la fisiología, incluida la capacidad de ejercicio, pero rara vez se han estudiado los efectos sobre el ejercicio voluntario.
Los antibióticos orales reducen el comportamiento de ejercicio voluntario en ratones atléticos
En un reciente estudio (https://doi.org/10.1016/j.beproc.2022.104650)
- Se criaron selectivamente líneas replicadas de ratones High Runner para el ejercicio voluntario.
- Se utilizó un cóctel de antibióticos de amplio espectro para reducir en gran medida el microbioma intestinal.
- Los antibióticos redujeron el comportamiento de funcionamiento de las ruedas, especialmente en los High Runners.
- Los antibióticos redujeron la duración del funcionamiento de las ruedas, pero no la velocidad.
- Los antibióticos no causaron enfermedad, según la masa corporal, el consumo de alimentos y agua.
EXPERIMENTO
Se estudió 4 líneas de ratones hembras High Runner (HR) replicadas para el ejercicio voluntario y se compararon con 4 líneas de control (C) no seleccionadas.
Los ratones HR y C difieren en varios rasgos que probablemente interactúan con el microbioma intestinal, incluida una mayor distancia diaria para correr, temperaturas corporales al correr, actividad física espontánea cuando se alojan sin ruedas y consumo de alimentos.
SUMINISTRO DE ANTIBIÓTICOS
Después de dos semanas de correr, para alcanzar una meseta estable en corridas diarias, a los ratones se les administraron antibióticos de amplio espectro durante 10 días.
El tratamiento con antibióticos provocó una reducción significativa en la distancia diaria de carrera en la rueda en los ratones HR (−21 %), pero no en los ratones C.
Figura1: Funcionamiento medio diario de la rueda (revoluciones por 23 h) a lo largo del experimento. Los valores se calcularon tomando las medias simples de las 4 líneas HR y las 4 líneas C para cada día individual y luego promediando esos valores para obtener el promedio de las líneas HR y C. Los datos se truncaron los días 8, 14 y 25 (~20 h) cuando se cambiaban las botellas de agua. Los corchetes indican los días que se utilizaron en los análisis estadísticos.
Los antibióticos no afectaron la masa corporal o el consumo de alimentos en ratones HR o C, y no observamos comportamiento de enfermedad .
El funcionamiento de la rueda de los ratones HR no se recuperó durante los 12 días posteriores al cese de los antibióticos. La disminución del funcionamiento de la rueda en los ratones HR pero no en los C, sin efectos secundarios negativos aparentes de los antibióticos, sugiere que el microbioma HR es un componente importante de su fenotipo de alto funcionamiento.
Los cócteles de antibióticos anteriores que incluían ampicilina , estreptomicina y neomicina en combinación con otros antibióticos han demostrado una disminución exitosa o el agotamiento de las colonias anaerobias.
La reducción del microbioma intestinal podría haber alterado la motivación y/o la capacidad para el ejercicio sostenido con apoyo aeróbico, los cuales son más altos en los ratones HR en relación con los ratones C.
Los antibióticos redujeron el consumo de alimentos en los ratones HR pero no en los C. Con la masa corporal como covariable, tanto los ratones HR como los C tuvieron un aumento significativo en el consumo de agua durante el tratamiento con antibióticos en comparación con los valores previos al antibiótico.
Efectos del tratamiento con antibióticos de amplio espectro o el estado libre de gérmenes sobre el rendimiento de resistencia y las adaptaciones al ejercicio en ratones
Se desconoce hasta qué punto los microbios intestinales son responsables de las adaptaciones fisiológicas al entrenamiento físico. En otro estudio reciente (DOI: 10.1249/MSS.0000000000003051) se intenta evaluar como el ejercicio de resistencia altera el microbioma intestinal.
PROPÓSITO
El propósito de estos experimentos fue determinar el papel de los microbios intestinales en el rendimiento y la adaptación muscular a seis semanas de carrera voluntaria sobre ruedas (VWR) en ratones.
MÉTODO
Eliminamos los microbios con un tratamiento con antibióticos de amplio espectro (ABX) y usamos ratones libres de gérmenes (GF) para determinar los efectos sobre las adaptaciones a VWR.
Se asignaron ratones macho y hembra C57Bl/6 (n = 56) a VWR diario a condiciones sedentarias. Después de la intervención, se evaluaron la resistencia a la cinta rodante y la tolerancia a la glucosa, y los tejidos del gastrocnemio y el sóleo. Se recolectaron y analizaron para determinar la actividad de la enzima citrato sintasa (CS) y la expresión de genes sensibles al entrenamiento físico.
RESULTADOS
El tratamiento con anfibióticos de alto espectro y el estado de los ratones libres de gérmenes (GF) redujeron el comportamiento de carrera voluntaria sobre ruedas (VWR), pero no afectaron las adaptaciones inducidas por VWR en la capacidad de resistencia, la actividad de la enzima citrato sintasa o la expresión de genes metabólicos musculares.
Sin embargo, el estado de GF redujo la capacidad de resistencia. Estos datos indicaron que la reducción de microbios en la edad adulta no inhibe las adaptaciones del entrenamiento de resistencia en ratones C57Bl/6.
CONCLUSIÓN
La conclusión práctica, según Hutchinson y sus colaboradores, es que si necesitas tomar antibióticos antes de una competencia importante, es poco probable que afecte tus adaptaciones de entrenamiento o su rendimiento. Creo que es una posición razonable y tranquilizadora, teniendo en cuenta todas las incertidumbres inherentes a la aplicación de estudios con ratones al comportamiento humano .
Por lo general, significa que es necesario realizar más investigaciones para averiguar si los hallazgos son reproducibles y aplicables a las otras condiciones. Lamentablemente, en la mayoría de los casos, la atención o el tratamiento no están disponibles de la noche a la mañana. Sin embargo, la (sobre)venta a menudo es necesaria para atraer nuevas subvenciones para la investigación. Dice el Dr. e Investigador Vootele Voikar, a cerca del uso de ratones en experimentos para la salud humana.
Pero es el aparente cambio en la motivación para hacer ejercicio lo que realmente intriga. ¿Hay algo allí que ayude a explicar quién de nosotros termina como High Runner? Si es así, ¿podemos manipularlo? Es fácil ver cómo podría quedar atrapado en el entusiasmo por los posibles nuevos suplementos probióticos que alteran no solo su capacidad sino también su deseo de hacer ejercicio. Garland, en un comunicado de prensa de UC Riverside , insinúa esa posibilidad. Pero su consejo, por ahora, está adecuadamente fundamentado en la realidad actual. Si desea un microbioma saludable, sugiere, debe comer una dieta balanceada y hacer ejercicio regularmente.
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Watch the top 100 of every year of each Money List since 1971 from The Hendon Mob and stacked them year after year to put them in a bar chart race. See some of the greatest rises and falls in poker history! more… An Eight Low is the weakest hand that qualifies for low in Omaha Hi Lo and Stud Hi Lo. However in Razz, there is no such ‘qualifier’ and the lowest hand will always win the pot, even if it is a nine low, queen low, or even a pair! It’s Saturday weekend ! And what a way to end the week ! Lately , I’ve been having more difficulty in one or two anagrams than the jumble solution ! And today , added to that ! The two anagrams that made me raise an eyebrow were DRAGON and SUPERB ! WEAVE was there with thread , and that led me to PUNCH it out ! The jumble was a royal flush and I did feel like the UPPER HAND ! Great way to end the week Saturday !!!!!
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PokerStars is the only place where you can watch and play against Team PokerStars Pro – our group of elite pro players. Between them they have scores of World Series bracelets, EPT and WPT titles and more, adding up to millions in winnings. And now you can join them at the tables with PokerStars. All texas Hold’em poker hands are divided into two categories: suited and offsuit. Suited poker hands are made of two cards of the same suit. For instance, K♥️ 10♥️, A♠️ K♠️, J♣️ 8♣️, 10♦️ 7♦️ are all suited hands. On the other hand, the Offsuit poker hands are made of two cards of different suits. For instance, K♦️ 7♣️, Q♥️ 9♦️, A♠️ 6♦️, 10♦️ 8♥️ are all offsuit hands. In middle to late position you want to play these hands with due diligence. You don’t want to be calling large raises to play these hands heads up. Your goal with these hands is to play the largest pots possible for the least amount of investment possible. You need great odds to make money on these. Without the odds, they should be folded from any position. In live texas holdem games you will experience players to play these kind of hands more often because you get dealt hands less often compared to online poker.
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Watch EVERY out-of-marketregular season gameLIVE or on demand in HD FA Cup final рџ™ЊрџЏј @ManUtd t.co hMdHumhY2I A point was enough to ensure Arsenal maintained their position at the top of the Premier League form table, with seven wins from their last ten outings, but City’s 3-1 win over Aston Villa at the Etihad Stadium means the gap at the top of the table is back down to three points and the gap in the form table to one. AFC Bournemouth For more information about these items, view our complete privacy policy.Read More Southampton, Nottingham Forest and Everton currently occupy the relegation places but that keeps changing all the time and it is so tight at the bottom of the table. Do you have a sports website? Or write about sports? We have tools and resources that can help you use sports data. Find out more.
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11 cities, 24 teams and hundreds of headaches: The European soccer championship is here after a year’s delay. Here’s what you need to know. We have just waved goodbye to a thrilling Euro 2020, but even though there are three years left before the next European Championship, let’s take a quick look at the upcoming Euro 2024. Final tournamentDecember 2023: UEFA EURO 2024 final tournament draw, Elbphilharmonie Hamburg14 June 2024: UEFA EURO 2024 opening game, Munich Football Arena14 July 2024: UEFA EURO 2024 final, Olympiastadion Berlin It may look like a UFO has landed next to the motorway in Munich but it is in fact the home of German giants Bayern Munich since 2005. Its outer scales light up red when Bayern are at home and white when the German national team plays here. Built for the 2006 World Cup, it will host the Euro 2024 opener on June 14 and one of the semifinals.
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